Interactions of calf-thymus histone fractions in aqueous solution with 8-anilinonaphthalene-1-sulphonic acid
نویسندگان
چکیده
منابع مشابه
Interactions of calf-thymus histone fractions in aqueous solution with 8-anilinonaphthalene-1-sulphonic acid.
1. The interactions of histone fractions with 8-anilinonaphthalene-1-sulphonic acid were investigated by fluorimetry and spectrofluorimetry and the results were interpreted with the aid of equilibrium-dialysis techniques. 2. Characteristic differences were found between the various histone fractions, and with fractions F3 and F2a the binding was found to be salt-dependent. 3. Evidence was obtai...
متن کاملComplete amino-acid sequence of calf-thymus histone 3.
Calf-thymus histone III is a single polypeptide chain of 135 residues (combined molecular weight of 15,324) with alanine at both the amino and carboxyl ends. The NH(2)-terminal region (Residues 1-53) of histone III is strongly basic (net charge of +18) and contains lysines-14 and -23, which are epsilon-N-acetylated in a fraction of the molecules, as well as lysines-9 and -27, which are partiall...
متن کاملChromatographic fractionation of calf thymus histone.
Evidence has recently accumulated that histone preparations, particularly those derived from the nuclei of calf thymus, may contain a mixture of different basic proteins. Variations in the amino acid composition of histone fractions extracted and precipitated by different means have been demonstrated by Stedman and Stedman (2), Daly, Mirsky, and Ris (3), Eadie and Leaf (4), Hamer (5), Davison a...
متن کاملStructure of the human-heart fatty-acid-binding protein 3 in complex with the fluorescent probe 1-anilinonaphthalene-8-sulphonic acid
Heart-type fatty-acid-binding protein (FABP3), which is a cytosolic protein abundantly found in cardiomyocytes, plays a role in trafficking fatty acids throughout cellular compartments by reversibly binding intracellular fatty acids with relatively high affinity. The fluorescent probe 1-anilinonaphthalene-8-sulfonate (ANS) is extensively utilized for examining the interaction of ligands with fa...
متن کاملThe inhibitory effects of calf thymus-histone fractions on the development of the chick embryo.
The genetic potentialities of an egg are transcribed, translated and built up into a temporal-spatial organization by epigenetic processes. How are these potentialities realized? Efforts to answer this question have naturally turned towards a study of the activity of the genetic material, which according to modern ideas is entirely composed of DNA. Stedmann & Stedmann (1950) originally suggeste...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1966
ISSN: 0006-2936
DOI: 10.1042/bj0990419